Wandless Lab



Ubiquitin-derived fragment as a peptide linker for the efficient cleavage of a target protein from a degron.
Y Utsugi, K Nishimura, S Yamanaka, K Nishino, H Kosako, T Sawasaki, H Shigemori, TJ Wandless, Y Miyamae.
ACS Chemical Biology 2024, 19, 497-505.


A cAMP Sensor Based on Ligand-Dependent Protein Stabilization.
M Sidoli, L-c Chen, AJ Lu, TJ Wandless, WS Talbot ACS Chemical Biology 2022, 17, 2024-2030.


Transient rest restores functionality in exhausted CAR-T cells through epigenetic remodeling.
E Weber, KR Parker, E Sotillo, RC Lynn, H Anbunathan, J Lattin, Z Good, JA Belk, B Daniel, D Klysz, M Malipatlolla, P Xu, M Bashti, S Heitzeneder, L Labanieh, P Vandris, RG Majzner, Y Qi, K Sandor, L-c Chen, S Prabhu, AJ Gentles, TJ Wandless, AT Satpathy, HY Chang, CL Mackall Science  2021, 372, eaba1786.


A Method for Conditional Regulation of Protein Stability in Native or Near-Native Form.
Y Miyamae, L-c Chen, Y Utsugi, H Farrants, TJ Wandless Cell Chemical Biology 2020, 27, 1573-1581.


A novel destabilizing domain based on a small-molecule dependent fluorophore.
Navarro R, Chen LC, Rakhit R, Wandless TJ. ACS Chemical Biology 2016 Aug 19;11(8):2101-4. doi: 10.1021/acschembio.6b00234.

A method to rapidly create protein aggregates in living cells.
Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, Wandless TJ. Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689.


Distinct transcriptional responses elicited by unfolded nuclear or cytoplasmic protein in mammalian cells.
Miyazaki Y, Chen LC, Chu BW, Swigut T, Wandless TJ. Elife. 2015 Aug 28;4. doi: 10.7554/eLife.07687.

Using light to shape chemical gradients for parallel and automated analysis of chemotaxis.
Collins SR, Yang HW, Bonger KM, Guignet EG, Wandless TJ, Meyer T. Mol Syst Biol. 2015 Apr 23;11(4):804. doi: 10.15252/msb.20156027.


Chemical biology strategies for posttranslational control of protein function.
Rakhit R, Navarro R, Wandless TJ. Chem Biol. 2014 Sep 18;21(9):1238-52. doi: 10.1016/j.chembiol.2014.08.011.

General method for regulating protein stability with light.
Bonger KM, Rakhit R, Payumo AY, Chen JK, Wandless TJ. ACS Chem Biol. 2014 Jan 17;9(1):111-5. doi: 10.1021/cb400755b.


The E3 ubiquitin ligase UBE3C enhances proteasome processivity by ubiquitinating partially proteolyzed substrates.
Chu BW, Kovary KM, Guillaume J, Chen LC, Teruel MN, Wandless TJ. J Biol Chem. 2013 Nov 29;288(48):34575-87. doi: 10.1074/jbc.M113.499350.

Inducible control of gene expression with destabilized Cre.
Sando R 3rd, Baumgaertel K, Pieraut S, Torabi-Rander N, Wandless TJ, Mayford M, Maximov A. Nat Methods. 2013 Nov;10(11):1085-8. doi: 10.1038/nmeth.2640.

Rapid and tunable control of protein stability in Caenorhabditis elegans using a small molecule.
Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ. PLoS One. 2013 Aug 22;8(8):e72393. doi: 10.1371/journal.pone.0072393. eCollection 2013.

Par-4 downregulation promotes breast cancer recurrence by preventing multinucleation following targeted therapy.
Alvarez JV, Pan TC, Ruth J, Feng Y, Zhou A, Pant D, Grimley JS, Wandless TJ, Demichele A; I-SPY 1 TRIAL Investigators, Chodosh LA. Cancer Cell. 2013 Jul 8;24(1):30-44. doi: 10.1016/j.ccr.2013.05.007.

FK506 activates BMPR2, rescues endothelial dysfunction, and reverses pulmonary hypertension.
Spiekerkoetter E, Tian X, Cai J, Hopper RK, Sudheendra D, Li CG, El-Bizri N, Sawada H, Haghighat R, Chan R, Haghighat L, de Jesus Perez V, Wang L, Reddy S, Zhao M, Bernstein D, Solow-Cordero DE, Beachy PA, Wandless TJ, Ten Dijke P, Rabinovitch M. J Clin Invest. 2013 Aug;123(8):3600-13. doi: 10.1172/JCI65592.

Visualizing cellular interactions with a generalized proximity reporter.
Sellmyer MA, Bronsart L, Imoto H, Contag CH, Wandless TJ, Prescher JA. Proc Natl Acad Sci U S A. 2013 May 21;110(21):8567-72. Doi: 10.1073/pnas.1218336110.


Networks of polarized actin filaments in the axon initial segment provide a
mechanism for sorting axonal and dendritic proteins. Watanabe K, Al-Bassam S, Miyazaki Y, Wandless TJ, Webster P, Arnold DB. Cell Rep. 2012 Dec 27;2(6):1546-53. doi: 10.1016/j.celrep.2012.11.015.

Intracellular context affects levels of a chemically dependent destabilizing domain.
Sellmyer MA, Chen LC, Egeler EL, Rakhit R, Wandless TJ. PLoS One. 2012;7(9):e43297. doi: 10.1371/journal.pone.0043297.

Differential trafficking of transport vesicles contributes to the localization of dendritic proteins.
Al-Bassam S, Xu M, Wandless TJ, Arnold DB. Cell Rep. 2012 Jul 26;2(1):89-100. doi: 10.1016/j.celrep.2012.05.018.

Destabilizing domains derived from the human estrogen receptor.
Miyazaki Y, Imoto H, Chen LC, Wandless TJ. J Am Chem Soc. 2012 Mar 7;134(9):3942-5. doi: 10.1021/ja209933r.


Imaging the impact of chemically inducible proteins on cellular dynamics in Vivo.
Leong HS, Lizardo MM, Ablack A, McPherson VA, Wandless TJ, Chambers AF, Lewis JD. PLoS One. 2012;7(1):e30177. doi: 10.1371/journal.pone.0030177.

Ligand-switchable substrates for a ubiquitin-proteasome system.
Egeler EL, Urner LM, Rakhit R, Liu CW, Wandless TJ. J Biol Chem. 2011 Sep 9;286(36):31328-36. doi: 10.1074/jbc.M111.264101.

Evaluation of FKBP and DHFR based destabilizing domains in Saccharomyces cerevisiae.
Rakhit R, Edwards SR, Iwamoto M, Wandless TJ. Bioorg Med Chem Lett. 2011 Sep 1;21(17):4965-8. doi: 10.1016/j.bmcl.2011.06.006.

Small-molecule displacement of a cryptic degron causes conditional protein degradation.
Bonger KM, Chen LC, Liu CW, Wandless TJ. Nat Chem Biol. 2011 Jul 3;7(8):531-7. doi: 10.1038/nchembio.598.

Asparagine repeat function in a Plasmodium falciparum protein assessed via a regulatable fluorescent affinity tag.
Muralidharan V, Oksman A, Iwamoto M, Wandless TJ, Goldberg DE. Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4411-6. doi: 10.1073/pnas.1018449108.

Chemical control of FGF-2 release for promoting calvarial healing with adipose stem cells.
Kwan MD, Sellmyer MA, Quarto N, Ho AM, Wandless TJ, Longaker MT. J Biol Chem. 2011 Apr 1;286(13):11307-13. doi: 10.1074/jbc.M110.180042. Epub 2011 Jan 24.


A general chemical method to regulate protein stability in the mammalian central nervous system.
Iwamoto M, Björklund T, Lundberg C, Kirik D, Wandless TJ. Chem Biol. 2010 Sep 24;17(9):981-8. doi: 10.1016/j.chembiol.2010.07.009.

A plant-like kinase in Plasmodium falciparum regulates parasite egress from erythrocytes.
Dvorin JD, Martyn DC, Patel SD, Grimley JS, Collins CR, Hopp CS, Bright AT, Westenberger S, Winzeler E, Blackman MJ, Baker DA, Wandless TJ, Duraisingh MT. Science. 2010 May 14;328(5980):910-2. doi: 10.1126/science.1188191.

Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae.
Edwards SR, Wandless TJ. Yeast. 2010 Apr;27(4):229-36. doi: 10.1002/yea.1744.


A general method for conditional regulation of protein stability in living animals.
Sellmyer MA, Thorne SH, Banaszynski LA, Contag CH, Wandless TJ. Cold Spring Harb Protoc. 2009 Mar;2009(3):pdb.prot5173. doi: 10.1101/pdb.prot5173. No abstract available.

Regulating protein stability in mammalian cells using small molecules.
Hagan EL, Banaszynski LA, Chen LC, Maynard-Smith LA, Wandless TJ. Cold Spring Harb Protoc. 2009 Mar;2009(3):pdb.prot5172. doi: 10.1101/pdb.prot5172. No abstract available.

The proteasome makes sense of mixed signals.
Wandless, TJ. Nat Chem Biol. 2009 Jan;5(1):3-4. doi: 10.1038/nchembio0109-3.


Recent progress with FKBP-derived destabilizing domains.
Chu BW, Banaszynski LA, Chen LC, Wandless TJ. Bioorg Med Chem Lett. 2008 Nov 15;18(22):5941-4. doi: 10.1016/j.bmcl.2008.09.043.

Chemical control of protein stability and function in living mice.
Banaszynski LA, Sellmyer MA, Contag CH, Wandless TJ, Thorne SH. Nat Med. 2008 Oct;14(10):1123-7. doi: 10.1038/nm.1754.

Synthesis and analysis of stabilizing ligands for FKBP-derived destabilizing domains.
Grimley JS, Chen DA, Banaszynski LA, Wandless TJ. Bioorg Med Chem Lett. 2008 Jan 15;18(2):759-61.


Rapid Control of Protein Levels in the Apicomplexan Parasite Toxoplasma gondii.
Herm-Gotz A, Agop-Nersesian C, Munter S, Grimley JS, Wandless TJ, Frischknecht F, Meissner M, Nature Methods, 2007

The enantioselective synthesis of phomopsin B.
Grimley JS, Sawayama AM, Tanaka H, Stohlmeyer MM, Woiwode TF, Wandless TJ. Angew Chem Int Ed Engl. 2007;46(43):8157-9. No abstract available.

A directed approach for engineering conditional protein stability using biologically silent small molecules.
Maynard-Smith LA, Chen LC, Banaszynski LA, Ooi AG, Wandless TJ. J Biol Chem. 2007 Aug 24;282(34):24866-72.

Rescue of degradation-prone mutants of the FK506-rapamycin binding (FRB) protein with chemical ligands.
Stankunas K, Bayle JH, Havranek JJ, Wandless TJ, Baker D, Crabtree GR, Gestwicki JE. Chembiochem. 2007 Jul 9;8(10):1162-9.

The rapamycin-binding domain of the protein kinase mammalian target of rapamycin is a destabilizing domain.
Edwards SR, Wandless TJ. J Biol Chem. 2007 May 4;282(18):13395-401. Epub 2007 Mar 9.

Engineering small molecule specificity in nearly identical cellular environments.
Sellmyer MA, Stankunas K, Briesewitz R, Crabtree GR, Wandless TJ. Bioorg Med Chem Lett. 2007 May 15;17(10):2703-5.


PI(3,4,5)P3 and PI(4,5)P2 lipids target proteins with polybasic clusters to the plasma membrane.
Heo WD, Inoue T, Park WS, Kim ML, Park BO, Wandless TJ, Meyer T. Science. 2006 Dec 1;314(5804):1458-61.

Plasma membrane dissociation of Ras superfamily small GTPases triggered by chemically-induced depletion of phosphoinositide lipid second messengers.
Heo, W. D.; Inoue, T.; Park, W. S.; Kim, M. L.; Park, B. O.; Wandless, T. J.; Meyer, T. Science, 2006, 314, 1458-1461.

A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.
Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. Cell. 2006 Sep 8;126(5):995-1004.

Rapamycin analogs with differential binding specificity permit orthogonal control of protein activity.
Bayle JH, Grimley JS, Stankunas K, Gestwicki JE, Wandless TJ, Crabtree GR. Chem Biol. 2006 Jan;13(1):99-107.

Conditional control of protein function.
Banaszynski LA, Wandless TJ. Chem Biol. 2006 Jan;13(1):11-21. Review.


A cell-permeable, activity-based probe for protein and lipid kinases.
Yee MC, Fas SC, Stohlmeyer MM, Wandless TJ, Cimprich KA. J Biol Chem. 2005 Aug 12;280(32):29053-9.

An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways.
Inoue T, Heo WD, Grimley JS, Wandless TJ, Meyer T. Nat Methods. 2005 Jun;2(6):415-8.

Characterization of the FKBP.rapamycin.FRB ternary complex.
Banaszynski LA, Liu CW, Wandless TJ. J Am Chem Soc. 2005 Apr 6;127(13):4715-21. Erratum in: J Am Chem Soc. 2006 Dec 13;128(49):15928.


Total synthesis of ustiloxin D and considerations on the origin of selectivity of the asymmetric allylic alkylation.
Sawayama AM, Tanaka H, Wandless TJ. J Org Chem. 2004 Dec 10;69(25):8810-20.

Quantitative analyses of bifunctional molecules.
Braun PD, Wandless TJ. Biochemistry. 2004 May 11;43(18):5406-13.


Conditional protein alleles using knockin mice and a chemical inducer of dimerization.
Stankunas K, Bayle JH, Gestwicki JE, Lin YM, Wandless TJ, Crabtree GR. Mol Cell. 2003 Dec;12(6):1615-24.

A bifunctional molecule that displays context-dependent cellular activity.
Braun PD, Barglow KT, Lin YM, Akompong T, Briesewitz R, Ray GT, Haldar K, Wandless TJ. J Am Chem Soc. 2003 Jun 25;125(25):7575-80.

Enantioselective total synthesis of ustiloxin D.
Tanaka H, Sawayama AM, Wandless TJ. J Am Chem Soc. 2003 Jun 11;125(23):6864-5.


Calcineurin inhibitors and the generalization of the presenting protein strategy.
Vogel KW, Briesewitz R, Wandless TJ, Crabtree GR. Adv Protein Chem. 2001;56:253-91. Review. No abstract available.


Mechanistic Studies of Affinity Modulation.
Rosen MK, Amos CD, Wandless TJ. J. Am. Chem. Soc., 2000, 122, 11979-11982.

A confederacy of bunches: fundamentals and applications of a self-associating protein.
Wandless TJ. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):6921-3. No abstract available.

Synthesis and Evaluation of Daunorubicin–Paclitaxel Dimers.
Kar AK, Braun, PD, Wandless TJ. Bioorg. Med. Chem. Lett., 2000, 10, 261-264.


An Efficient Synthesis of β,γ-Dehydrovaline.
Woiwode TF, Wandless, TJ. J. Org. Chem. 1999, 64, 7670-7674

A Stereospecific Elimination to Form Dehydroamino Acids: Synthesis of the Phomopsin Tripeptide Sidechain.
Stohlmeyer MM, Tanaka H, Wandless TJ. J. Am. Chem. Soc., 1999, 121, 6100-6101

Affinity modulation of small-molecule ligands by borrowing endogenous protein surfaces.
Briesewitz R, Ray GT, Wandless TJ, Crabtree GR. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1953-8.


A Simple and Efficient Method for the Preparation of Hindered Alkyl–Aryl Ethers.
Woiwode TF, Rose C, Wandless TJ. J. Org. Chem. 1998, 63, 9594-9596


SH2 domains: a question of independence.
Wandless TJ. Curr Biol. 1996 Feb 1;6(2):125-7. Review.